Principle and procedure for fungal staining by editorial team on january 11, 2020 in mycology the lactophenol cotton blue lpcb wet mount is most widely used method for the staining and observation of fungi. Wet mount preparation is the most widely used method of. Lactophenol cotton blue wet mount is simple and widelthe y. Identification of the substancemixture and of the companyundertaking 1. Short video of using a lactophenol cotton blue stain for identifying fungal elements on a glass slide.
Lactophenol blue solution is a mounting medium and staining agent used in the preparation of slides for microscopic examination of fungi. The lactophenol cotton blue abbreviated lpcb stain wet mount preparation is one of the most widely used method of staining and observing fungi because it is simple to prepare. Lactophenol cotton blue stain definition of lactophenol. Lactophenol cotton blue according to regulation ec no 19072006, annex ii, as amended by regulation eu no 4532010 section 1. Fungi are eukaryotic organism and they are classified into two main groups that is yeast and molds. Product identifier product name lactophenol cotton blue product number pl. Gram stain and lactophenol cotton blue stain are used to visualize certain fungal structures that are significant.
Lactophenol cotton blue is a blue stain used for direct examination of clinical specimens for fungal elements. Lactophenol cotton blue reagent is used for fungal staining. Lactophenol along with cotton blue reagent is used for staining as well as for wet mounting of fungi1. Use of safe work practices are recommended to avoid eye or skin contact and inhalation of vapours. Pdf evaluation of lactophenol cotton blue for wet mount.
I read some of journal and they used lactophenol cotton blue stain but its hard to find it here. Our lactophenol blue stain is used as a mounting medium for studying fungi. Apart from lactophenolcotton blue, haematoxylin, melzers reagent,lactophenol. Lactophenol anilin blue solution, lactophenol cotton blue solution. Lactophenol cotton blue stain principle and interpretation. Pdf the use of lactophenol cotton blue lpcb stain for wet mount preparation of stools to demonstrate intestinal parasites by routine. Poly bottle diagnostic tests and clinical products. Lactophenol cotton blue stain droppers becton dickinson. Lactophenol blue solution for microscopy, for staining. Staining of fungus by using lactophenol cotton blue. Lactophenol blue solution for microscopy, for staining molds. The lactophenol cotton blue lpcb wet mount preparation is the most.
Fungus are eukaryotic organism and they are classified into two main groups that is yeast and molds. To perform tests, squeeze the dropper to crush the ampule. To stain fungal cells by using lactophenol cotton blue. What safe alternatives are there to lactophenol cotton. Lactophenol cotton blue solution is a mounting medium and staining agent used in the preparation of slides for microscopic examination of fungi. I read some of journal and they used lactophenol cotton blue stain but. Staining fungal structures with artificial dyes used in. Invert the dropper for convenient dropbydrop dispensing.
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